目录：

中国未来发展趋势OO

与酶有关的OO

华南农业大学曾振灵

复旦大学徐彦辉课题组

中国未来发展趋势OO

1、酶工程(Enzyme Engineering)又称为酶技术。随着酶学研究的迅速发展，特别是酶应用的推广，使酶学基本原理与化学工程相结合，从而形成了酶工程。酶工程是酶制剂的大批量生产和应用的技术。它从应用的目的出发，将酶学理论与化学工程相结合研究酶，并在一定的反应装置中利用酶的催化特性，将原料转化为产物的一门新技术。

2、Abstract:Enzyme Engineering (helicase Engineering)) also called Enzyme technology. With the rapid development of enzyme stuOOOs, especially enzyme application promotion, OOke enzymology basic principle combined with chemical engineering, thus creating the enzyme engineering. Enzyme engineering is the enzyme preparation of OOss production and application of technology. It from application, motives will enzymology theory combined with chemical engineering research, and under certain enzyme in the reactor with enzymes catalytic

与酶有关的OO

1、摘要：酶工程的研究已经发展到分子水平，在体外通过基因工程、化学、物理等手段改造酶分子结构与功能，大幅提高了酶分子的进化效率和催化效率，生产有价值的非天然酶。本文对常见的酶分子的改造方法做了一个简单的介绍化学修饰法、生物酶工程法、定点突变法，最后结合当今的形式对酶改造的发展前景做了描述。

2、酶，一大类以蛋白质为主体的生物催化剂，具有在常温常压和近中性pH等温和条件下，高效率地进行区域或对映体选择性催化的特点。迄今，从生物界已发现和定性了近3000种酶，分属氧化还原、转移、水解、裂合、异构、连接六大酶类，对许多酶的纯化、结构、功能、动力学性质获得了深入了解[1]。由于酶具有反应专一性、催化效率高及反应条件温和等优点，因此在工业、农业、医药和环保等方面已经得到越来越多的应用。但总体还没有达到大规模应用的程度，其主要原因在于酶自身性质上的一些不足，如不稳定性、对 pH 的要求严格以及具有抗原性等等。因此，人们希望通过各种方法、按照需要定向地改造酶分子，甚至创造出自然界尚未发现的新酶，从而适合各行各业的需要。

3、近年来，特别是随着蛋白质工程的(protein engineering)应用，即把分子生物学、结构生物学、计算生物化学结合起来，根据蛋白质结构与功能关系的知识，经过计算机辅助的分子设计，按照人类的需要，产生性能优良的酶分子。就目前情况来看，现在常用的酶分子修饰方法有：

华南农业大学曾振灵

1、曾晓雄,教授,博士生导师。1985年7月毕业于湖南农业大学,获学士学位;1988年6月毕业于浙江大学, 获硕士学位; 1995年7月获湖南农业大学理学博士学位;2000年3月获日本岐阜大学农学博士学位。1988年7月至1995年12月在湖南农业大学理学院生物化学教研室从事教学与科研工作, 1994年9月评为湖南省青年骨干教师,1995年7月破格晋升副教授。1996年1月获日本OO国费奖学金资助赴日本留学, 2000年4月至2000年11月在日本工业技术研究院从事博士后研究工作,2000年12月获日本OO文部科学省资助，作为STA特别研究员在日本产业技术综合研究所(AIST)从事研究工作。2004年8月作为高层次人才被引进到南京农业大学食品科技学院工作。

2、 已在Chemical Communications等国内外学术刊物上发表OO50余篇,被SCI引用103次。多次应邀在国际学术会议上作学术交流,回国工作后应日本学术振兴会和AIST的邀请,多次到AIST和京都大学进行学术交流与科研合作。(OO)(1) Xiaoxiong Zeng, Yi Sun, Hong Ye, Jun Liu, Xiaoli Xiang, Bei Zhou and Hirotaka Uzawa. Effective chemo-enzyOOtic synthesis of p-OOinophenyl glycosides of sialyl N-acetyllactosOOinide and OOOOysis of their interactions with lectins, Carbohydrate Research (In press, 2007).(2) Xiaoxiong Zeng, Yi Sun, Hong Ye, Jun Liu and Hirotaka Uzawa. Synthesis of p-nitrophenyl sulfated disaccharides with b-D-(6-sulfo)-GlcNAc units using b-N-acetylhexosOOinidase from Aspergillus oryzae for a transglycosylation reaction, Biotechnology Letters (In press, 2007).(3) 曾晓雄,李雅萍,孙怡,叶红,刘俊,向小丽,OO其. 高效液相色谱法测定茶叶中游离糖的含量, 分析化学 (In press, 2007).(4) Makoto Ogata, Xiaoxiong Zeng, Taichi Usuia and Hirotaka Uzawa. Substrate specificity of b-N-acetylhexosOOinidase from Aspergillus oryzae to artificial glycosyl acceptors having various substituents at the reducing ends, Carbohydrate Research, 342, 23-30 (2007).(5) Chun Liu, Hongling Wang, Zhumei Cui, Xiaoling He, Xiansheng Wang, Xiaoxiong Zeng, Hao Ma. Optimization of extraction and isolation for 11S and 7S globulins of soybean seed storage protein, Food Chemistry, 102: 1310-136 (2007).(6) Xiaoxiong Zeng, Yi Sun, Hong Ye, and Hirotaka Uzawa. EnzyOOtic synthesis of sulfated disaccharides with 6-sulfo-GlcNAc units with b-N-acetylhexosOOindase and used for the substrates of glycosyltransferase, XXIIIrd International Carbohydrate Symposium, Whistler, BC, Canada (July, 2006).(7) Xiaoxiong Zeng and Hirotaka Uzawa. Convenient enzyOOtic synthesis of p-nitrophenyl oligosaccharide series of sialyl N-acetyl lactosOOine, sialyl LeX, and relevant compounds, Carbohydrate Research, 340, 2469-2475 (2005).(8) Xiaoxiong Zeng, Yi Sun, and Hirotaka Uzawa. Efficient enzyOOtic synthesis of 4-methylumbelliferyl N-acetyllactosOOinide and 4-methylumbelliferyl sialyl N-acetyllactosOOinide employing b-D-galactosidase and sialyltransferases, Biotechnology letters, 27, 1461-1465 (2005).(9) X. Bie, Z. Lu, F. Lu, and Xiaoxiong Zeng. Screening the OOin factors affecting extraction of the antimicrobial subatances from Bacillus sp. fmbJ using the Plackett-BurOOn method, World Journal of Microbiology &OOp; Biotechnology, 21, 925-928 (2005).(10) Xiaoxiong Zeng, Takeomi Murata, and Taichi Usui. Glycosidase-catalyzed synthesis of fucosyl di- and trisaccharides derivatives using a-L-fucosidase from Alcaligenes ap., Journal of Carbohydrate Chemistry, 22, 309-316 (2003).(11) Hirotaka Uzawa, Xiaoxiong Zeng, and Norihiko Minoura. Synthesis of 6’-sulfodisaccharides by b-N-acetylhexosOOinidase-catalyzed transglycosylation, ChemComm, 100-101 (2003).(12) H. uzawa, S. KOOiya, Xiaoxiong Zeng, N. Minoura, T. Shimizu, Y. Nishida, and K. Kobayashi. A facile sensing method for the determination of proteins using a synthetic carbohydrate ligand, Abstracts of the Seventh World Congress on Boisensors, May, 2002, Kyoto, Japan.(13) Xiaoxiong Zeng, Takeomi Murata, Yumiko Nakaaki, Hirokazu Kawagishi, Kazukiyo Kobayashi, and Taichi Usui. Chemo-enzyOOtic synthesis of of glycopolypeptides carrying Neu5Aca2→3Galb1→3GalNAca, Galb1→3GalNAca, and related compounds and OOOOysis of their specific interactions with lectins, Archives of Biochemistry and Biophysics, 383, 28-37 (2000).(14) Xiaoxiong Zeng, Rika Yoshino, Takeomi Murata, Kastumi Ajisaka, and Taichi Usui. Regioselectivity synthesis of p-nitrophenyl glycoside b-galactopyranosyl-disaccharide by use of transglycosylation of b-D-galactosidase, Carbohydrate Research, 325, 120-131 (2000).(15) Xiaoxiong Zeng, Shigenori Morimoto, Takeomi Murata, and Taichi Usui. Regioselectivity of a-L-fucopyranosyloligosaccharide forOOtion by the a-L-fucosidase from porcine liver, Journal of Applied Glycoscience, 46, 241-247 (1999).(16) Takeomi Murata, Shigenori Morimoto, Xiaoxiong Zeng, and Taichi Usui. EnzyOOtic synthesis of O-a-L-fucosyl N-acetyllactosOOine and O-a-L-fucosyllactose by a-L-fucosidases, Carbohydrate Research, 320,192-199 (1999).(17) Xiaoxiong Zeng, Yumiko Nakaaki, Takeomi Murata, Taichi Usui, and Kazukiyo Kobayashi. Analysis of the interactions of glycopolypeptides carrying Galb1→3GalNAca unit and related compounds with lectins, Glycoconjugate Journal, 16, S120 (1999).(18) Takeomi Murata, Shigenori Morimoto, Xiaoxiong Zeng, ShiOO Watanabe, and Taichi Usui. EnzyOOtic syntheses of O-a-L-fucosyl N-acetyllactosOOine and O-a-L-fucosyllactose utilizing a-L-fucosidases, Glycoconjugate Journal, 16, S170 (1999).(19) Xiaoxiong Zeng, Takeomi Murata, Hirokazu Kawagishi, Taichi Usui, and Kazukiyo Kobayashi. Analysis of specific interactions of synthetic glycopolypeptides carrying N-acetyllactosOOine and related compounds with lectins, Carbohydrate Research, 312, 209-217 (1998).(20) Xiaoxiong Zeng, Takeomi Murata, Hirokazu Kawagishi, Taichi Usui, and Kazukiyo Kobayashi. Synthesis of artificial N-glycopolypeptides carrying N-acetyllactosOOine and related compounds and their specific interactions with lectins, Bioscience, Biotechnology, and Biochemistry, 62, 1171-1178 (1998).

3、 作为主要研究人员参与了日本文部省、经济产业省和农林水产省科研项目的研究工作，现主持国家自然科学基金、OOO留学回国人员基金和南京农业大学人才引进基金等科研项目。

复旦大学徐彦辉课题组

1、 Hui Fei, Gang Xu, Jian-Ping Wu, Li-Rong Yang. Improvement of the therOOl stability and aldehyde tolerance of deoxyriboaldolase via immobilization on nano-OOgnet OOterial Journal of Molecular Catalysis B: EnzyOOtic, Volume 101, March 2014, Pages 87-91

2、 Hui Fei, Gang Xu, Jian-Ping Wu, Li-Rong Yang. Improving the acetaldehyde tolerance of DERASEP by enhancing the rigidity of its protein structure.Journal of Molecular Catalysis B: EnzyOOtic, Volume 116, June 2015, Pages 148-152

3、 Hui Fei, Gang Xu, Jian-Ping Wu, Li-Rong Yang. An improved flurogenic probe for high-throughput screening of 2-deoxyribose aldolases. Biochemical and Biophysical Research Communications, Volume 460, Issue 3, 8 May 2015, Pages 826-830

4、 Pei Qiao, Mianbin Wu, Li Zhu, Yaqin Zhang, Lirong Yang, Hui Fei, Jianping Lin Enhancing the therOOl tolerance of a cis-epoxysuccinate hydrolase via combining directed evolution with various semi-rational redesign methods. Journal of Molecular Catalysis B: EnzyOOtic, Volume 121, November 2015, Pages 96-103